Aedes aegypti (Aedes aegypti)

Aedes aegypti, commonly known as the Yellow fever mosquito is the primary mosquito vector responsible for the transmission of both the yellow fever and dengue fever viruses. The Aedes aegypti Liverpool (LVP) strain sequenced originated in West Africa and has been maintained at the Liverpool School of Tropical Medicine since 1936. It was selected for susceptibility to the filarial worm Brugia malayi, and then subsequently a substrain (LVP^sbm) selected for greater Brugia susceptibility. Genome sequencing was performed on DNA purified from newly hatched larvae of an LVP^sbm strain derived after 12 consecutive generations of single pair inbreeding. This inbred strain has been designated Aedes aegypti LVP^1b12.

Assembly

The Aedes aegypti Liverpool LVP strain genome sequence is a joint effort between the Broad Institute and The Institute for Genomic Research (TIGR). Assembly of 8x shotgun coverage was performed using the Broad's whole genome assembly package ARACHNE. The assembly presented here (AaegL1, August 2005) consists of 4,758 supercontigs, totalling 1.3 Gigabases, with a Contig N50 of 82 Kb and supercontig N50 size of 1,500 Kb.

Annotation

The initial annotation of the Aedes genome is a collaboration between VectorBase and TIGR. Each group generated a set of gene predictions which were merged into a single canonical set (AaegL1.1). See the brief overview for details of how each set was generated. The geneset presented here (AaegL1.2, September 2009) represents the original set with the integration of community annotation and inclusion of additional gene predictions which were excluded from the AaegL1.1 set but subsequently found to have supporting evidence (transcriptome, mapped protein domains).

Genome Statistics