Culex quinquefasciatus gene set annotation

Organisms: 
Culex quinquefasciatus
Strains: 
Johannesburg
Assemblies: 
CpipJ2
Gene build id: 
CpipJ2.2
Description: 
Genes were annotated by merging VectorBase, JCVI and BROAD prediction sets. The VectorBase automatic analysis pipeline used either a GeneWise/Exonerate model from a known protein or a set of aligned cDNAs/ESTs followed by an ORF prediction. GeneWise/Exonerate models are further combined with available aligned cDNAs/ESTs to annotate UTRs (for more information see V.Curwen et al., Genome Res. 2004 14:942-50). JCVI annotations were based on Augustus (M. Stanke), GlimmerHMM (M. Pertea), Twinscan (M. Brent), Snap (I. Korf), and PHAT (S. Cawley) gene predictions, coupled with protein and EST genome alignments (PASA (M. Campbell), Genewise (E. Birney) and nap (X. Huang)). BROAD annotations were based on a combination of Augustus, GeneID (R. Guigo), FGeneSH (A. Salamov), GeneWise, and SNAP, and completed by species specific protein alignments (BLAST).