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RNA-Seq Experiment Ivermectin-containing blood meals at different ages (Seaman et al., 2015)
NameIvermectin-containing blood meals at different ages (Seaman et al., 2015)
SpeciesAnopheles gambiae
Experimental factorsAge:GrowthCondition:Compound
Statistical testsANOVA
DescriptionGene expression was compared between blood-fed and non-blood-fed adult female Anopheles gambiae mosquitoes. Furthermore, the blood meals either contained 11.75 ng/ml ivermectin (solubilized in dimethyl sulfoxide and diluted in PBS) or the same volume of DMSO alone in PBS as control. The first blood meal was at two days post-emergence and a second blood meal was administered at six days post-emergence. The non-blood-fed control at six days did have the blood meal at two days. Mosquitoes receiving an ivermectin treated blood at six days were given an untreated blood meal at two days. All mosquitoes had ad libitum access to sugar and water except when being starved for several hours on blood-feeding days. Transcript expression was assayed via paired-end Illumina sequencing with quantification through a simple tophat2/cufflinks pipeline.
AuthorsJonathan A. Seaman, Haoues Alout1, Jacob I. Meyers, Mark D. Stenglein, Roch K. Dabiré,Saul Lozano-Fuentes, Timothy A. Burton, Wojtek S. Kuklinski, William C. Black IV, Brian D. Foy
AffiliationsArthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University 1692 Campus Delivery, Fort Collins, CO 80525, USA Institute de Recherche en Sciences de la Santé (IRSS)/Centre Muraz, Direction Régionale de l’Ouest, 399 Ave de la Liberté, Bobo Dioulasso, Houet, Burkina Faso 10400-000
Accessions SRA: SRP060547
PubMed ID 26471037
Search expression data from this experiment
Genesets used
Geneset Description
Anopheles gambiae AgamP4.2 transcripts A reference set of transcripts for the quantification of expression (FPKM) using Cufflinks. Visit https://www.vectorbase.org/organisms/anopheles-gambiae/pest/AgamP4.2

Sequencing Runs
12 items found, displaying all items.
Name Samples
2DPE_NBF_1.s1.e1.ampl 2DPE_NBF_1.s1
2DPE_NBF_2.s1.e1.ampl 2DPE_NBF_2.s1
2DPE_BF_1.s1.e1.ampl 2DPE_BF_1.s1
2DPE_BF_2.s1.e1.ampl 2DPE_BF_2.s1
2DPE_IVM_1.s1.e1.ampl 2DPE_IVM_1.s1
2DPE_IVM_2.s1.e1.ampl 2DPE_IVM_2.s1
6DPE_NBF_1.s1.e1.ampl 6DPE_NBF_1.s1
6DPE_NBF_2.s1.e1.ampl 6DPE_NBF_2.s1
6DPE_BF_1.s1.e1.ampl 6DPE_BF_1.s1
6DPE_BF_2.s1.e1.ampl 6DPE_BF_2.s1
6DPE_IVM_1.s1.e1.ampl 6DPE_IVM_1.s1
6DPE_IVM_2.s1.e1.ampl 6DPE_IVM_2.s1
12 items found, displaying all items.