Bioinformatics Resource for Invertebrate Vectors of Human Pathogens
All Microarrays and Genesets
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MMC1 is a spotted cDNA microarray. It includes a total of 18,760 sequenced Anopheles EST clones prepared from two immune competent cell lines (Dimopoulos et al., 2000; Dimopoulos et al., 2001) and pooled mosquito developmental stages and adult mosquito heads (G. K. Christophides, unpublished). ArrayExpress: A-MEXP-225

The Anopheles 4K DNA microarray platform contains 3,840 EST clones prepared from two immune competent cell lines (Dimopoulos et al., 2000; Dimopoulos et al., 2001). All 4K ESTs are also spotted on the MMC1 array. The v1.0 and v2.0 arrays differ only in the spotting order. ArrayExpress: A-MEXP-226

The Anopheles 4K DNA microarray platform contains 3,840 EST clones prepared from two immune competent cell lines (Dimopoulos et al., 2000; Dimopoulos et al., 2001). All 4K ESTs are also spotted on the MMC1 array. The v1.0 and v2.0 arrays differ only in the spotting order. ArrayExpress: A-MEXP-227

The MMC2 microarray is a spotted PCR amplicon array. The amplicons are 150-500 bp long and mostly represent single exons located preferably close to the 3' ends of coding sequences of 12,500 predicted genes in the A. gambiae genome.

A total of 9,600 total features were printed. Out of these features, 9,504 were cDNA clones from Aedes aegypti and 96 were buffer blanks. ArrayExpress: A-MEXP-940

A spotted oligo array containing fragments of 235 members of the cytochrome P450, glutathione transferase and carboxy/cholinesterase families in Aedes aegypti. ArrayExpress: A-MEXP-623

A microarray containing fragments of 230 Anopheles gambiae genes from families associated with metabolic-based insecticide resistance was constructed. These gene fragments included 103 cytochrome P450s, 31 esterases, 35 GSTs, 41 Red/Ox genes, 5 ABC transporters, tissue specific genes and housekeeping genes. Each gene represented on the microarray was either obtained by PCR amplification or artificially synthesized as a 70-mer antisense oligo. ArrayExpress: A-MEXP-137

A microarray containing fragments of 254 Anopheles gambiae genes from families associated with metabolic-based insecticide resistance was constructed. These gene fragments included 103 cytochrome P450s, 31 esterases, 35 glutathione S-transferases, 47 red/ox genes, 5 ATP-binding cassette transporters, tissue specific genes and housekeeping genes. Each gene represented on the microarray was either obtained by PCR amplification or artificially synthesized as a 70-mer oligo. ArrayExpress: A-MEXP-863

A spotted oligo array containing fragments of 312 members of the cytochrome P450, glutathione transferase and carboxy/cholinesterase families in Aedes aegypti.

60-mer oligomers designed for 1928 EST clusters generated for immune-response activated hemocytes from adult Ae. aegypti females by the Bruce Christensen Laboratory, University of Wisconsin-Madison. The arrays have 4 features per reporter. GEO: GPL2994

60-mer oligomers designed for 1928 EST clusters generated for immune-response activated hemocytes from adult Ae. aegypti females by the Bruce Christensen Laboratory, University of Wisconsin-Madison. The arrays have 4 features per reporter. Print variant of v1. GEO: GPL2982

Custom-commercial Aedes aegypti array from Agilent, containing 45220 60-mer probes, designed against the AaegL1.1 set of 16k transcripts. GEO: GPL8972

Custom-commercial Anopheles gambiae array from Agilent, containing 60-mer probes designed against 13k transcripts from the AgamP3.4 gene build. GEO: GPL9246

Custom-commercial Aedes aegypti array from Agilent, containing 45220 60-mer probes, designed against the AaegL1.1 set of 16k transcripts at the University of California, Irvine. GEO: GPL10542

This oligo DNA array was designed at the National Institute of Infectious Diseases, Japan, for 62 different cDNA segments encoding unique P450 isoforms of the Culex pipiens complex. Other probes for non-P450 Culex pipiens complex genes were also designed. The array uses Agilent's flexible SurePrint in situ synthesis. GEO: GPL8743.

Custom arrays were designed based on the Roche NimbleGen 12-plex format (12 arrays per slide with 135 000 60-mer probes per array). The design includes at least five probes per gene, synthesized in duplicate, providing a total of 131 212 probes that interrogate the 13 254 genes annotated in the AgamP3.5 genebuild. This array is available through NimbleGen (catalogue number OID22384; design name 090706_A_gambiae_NotreDame_BH_expr_HX12) and ArrayExpress: A-MEXP-1954

This is a 8 x 15K spotted glass array constructed using Agilent's Spotted Microarray Technology platform. The chip was spotted with 60 nucleotide sequences of Aedes aegypti transcripts (AaegL1.2, September 2009). The probes were checked against the Ae. aegypti and Homo sapiens genomes for cross-hybridization, any that cross-hybridized were removed from the chip. Approximately 15,032 transcripts are represented by a single probe in a randomised layout. Each of the eight arrays is an identical replicate. ArrayExpress: A-MEXP-1966

Commercially available Anopheles gambiae array from Agilent. 43,803 60-mer probes sourced from RefSeq, UniGene, TIGR, Ensembl, Entrez gene, anoGarn1 and UCSC dm3. GEO: GPL13157 This version has a different layout compared with the earlier version.

This is a 4 x 44K spotted glass array constructed using Agilent Spotted Microarray Technology platform. The chip was spotted with 60 nucleotide sequences of Aedes aegypti transcripts. The probe sequences were checked against the Ae. aegypti and Homo sapiens genomes for cross-hybridization, any that cross-hybridized were removed from the chip. There were approximately 13,500 aedes transcripts used. Each is replicated three times per array.

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