Bioinformatics Resource for Invertebrate Vectors of Human Pathogens
We are excited to announce that VectorBase and EuPathDB are now one bioinformatic resource center. See the press release and our letter to the community to learn more.
Microarray EMBL A. gambiae MMC1 20k v1.0
Description MMC1 is a spotted cDNA microarray. It includes a total of 18,760 sequenced Anopheles EST clones prepared from two immune competent cell lines (Dimopoulos et al., 2000; Dimopoulos et al., 2001) and pooled mosquito developmental stages and adult mosquito heads (G. K. Christophides, unpublished). ArrayExpress: A-MEXP-225
Accessions ArrayExpress: A-MEXP-225
Search for probes from this platform
Experiments
Name Description
Developmental series (Koutsos et al., 2007) Seven different Anopheles gambiae developmental stages (from embryo to adult) are profiled using the MMC1 EST array. The larval samples are time-based (rather than instar-based).

The expression ratios are calculated with respect to a standard reference sample in channel 2. The ratios can not be interpreted as up- or down-regulated. They may only be compared relative to each other.

Male vs. female (Koutsos et al., 2007) Adult male and female Anopheles gambiae samples are profiled using the MMC1 EST array.

The expression ratios are calculated with respect to a standard reference sample in channel 2. The ratios can not be interpreted as up- or down-regulated. They may only be compared relative to each other.

Plasmodium berghei midgut invasion time-series (Vlachou et al., 2005) Measures the Anopheles gambiae midgut's response to Plasmodium berghei invasion at three time points: before, during and after midgut invasion. The control uses an invasion-deficient strain thereby eliminating the effects of blood feeding.

The MMC1 EST array is used.

Plasmodium berghei midgut invasion stage comparisons (Vlachou et al., 2005) Changes in expression between neighboring stages of Anopheles gambiae midgut invasion are measured separately for wild-type and invasion-deficient strains of Plasmodium berghei using the MMC1 EST array.

Look for changes in the wild-type experiments that are not mirrored by the invasion-deficient control.

Adult female tissues (Koutsos et al., 2007) Four different Anopheles gambiae adult body parts (head, midgut, ovaries and carcass) are profiled using the MMC1 EST array. The carcass sample is what remains after head, midgut and ovaries have been dissected.

The expression ratios are calculated with respect to a standard reference sample in channel 2. The ratios can not be interpreted as up- or down-regulated. They may only be compared relative to each other.

Chloroquine exposure (Abrantes et al., 2008) Midguts from uninfected and Plasmodium berghei infected Anopheles gambiae mosquitoes were exposed to the anti-malarial drug chloroquine or a control. The MMC1 EST-based array was used.