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Microarray Affymetrix Plasmodium Anopheles
Description The GeneChip® Plasmodium/Anopheles Genome Array includes probe sets to over 4,300 Plasmodium falciparum transcripts and approximately 14,900 Anopheles gambiae transcripts. The Plasmodium/Anopheles Genome Array was developed in collaboration with the Malaria Research Institute at the Johns Hopkins Bloomberg School of Public Health. more
Accessions GEO: GPL1321
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Experiments
Name Description
Larval and adult stages (Marinotti et al., 2006) Larval and adult stages of Anopheles gambiae are compared using the Affymetrix chip. (Adult male and female samples have been averaged in the analysis.)
Blood meal time series (Marinotti et al., 2006) Three day old Anopheles gambiae females are given a blood meal and are profiled at 7 different time points using the Affymetrix chip. This dataset can also be queried at http://www.angaged.bio.uci.edu/
Blood meal after 15 days (Marinotti et al., 2006) Comparison of 15 day uninfected blood-fed vs. sugar-fed Anopheles gambiae females using the Affymetrix chip.
Two consecutive blood meals (Marinotti et al., 2006) Two consecutive blood meals are profiled in Anopheles gambiae adult females with the Affymetrix chip. The second and third samples are taken 24h after each blood meal. The second blood meal is 48h after the first.
Male vs. female (Marinotti et al., 2006) Comparison of non-blood fed adult Anopheles gambiae females with males of same age using the Affymetrix chip.
Blood-fed adult female tissues (Marinotti et al., 2006) Three tissues (midgut, fat body and ovaries) of blood-fed adult Anopheles gambiae females are profiled using the Affymetrix chip. This dataset can also be queried at http://www.angaged.bio.uci.edu/
Larval salivary glands (Neira Oviedo et al., 2009) The expression profiles of Anopheles gambiae larval salivary glands vs. whole larvae are compared using the Affymetrix Anopheles/Plasmodium microarray.
Alimentary canal compartments (Neira Oviedo et al., 2008) Four different sub-compartments of the Anopheles gambiae larval midgut are profiled with the Affymetrix chip. A whole organism sample is also profiled for comparison.
M and S form virgin females (Cassone et al., 2008) We examined patterns of gene expression in two independent colonies of both M and S molecular forms of sugar fed virgin female Anopheles gambiae adults. For each colony, replicates were derived from independent RNA samples extracted from different cohorts to ensure that trends were reproducible. In addition, each replicate was derived from adults drawn from three cages to minimize the contribution of any individual cage to variation between samples. Data were obtained from a total of five biological replicates per mosquito colony.
M and S form 4th instar larvae (Cassone et al., 2008) We examined patterns of gene expression in two independent colonies of both M and S molecular forms of 4th instar Anopheles gambiae larvae. For each colony, replicates were derived from independent RNA samples extracted from different cohorts to ensure that trends were reproducible. In addition, each replicate was derived from larvae drawn from three pans to minimize the contribution of any individual pan to variation between samples. Data were obtained from a total of five biological replicates per mosquito colony.
M and S form gravid females (Cassone et al., 2008) We examined patterns of gene expression in two independent colonies of both M and S molecular forms of gravid female Anopheles gambiae adults. For each colony, replicates were derived from independent RNA samples extracted from different cohorts to ensure that trends were reproducible. In addition, each replicate was derived from adults drawn from three cages to minimize the contribution of any individual cage to variation between samples. Data were obtained from a total of five biological replicates per mosquito colony.
Mated females (Rogers et al., 2009) Microarray experiments comparing Anopheles gambiae virgins with females at 2 h, 6 h, and 24 h after mating were performed using the Affymetrix Anopheles/Plasmodium array.
Embryonic development (Goltsev et al., 2009) Gene expression in Anopheles gambiae embryos has been profiled at many time points using the Affymetrix Anopheles/Plasmodium microarray. Time points 49h and 52h have been removed due to lack of replicates.
Embryonic serosa (Goltsev et al., 2009) Gene expression profiles of the Anopheles gambiae embryo and serosa (shell) are compared using the Affymetrix Anopheles/Plasmodium microarray.
Hemolymph, carcass and head (Pinto et al., 2009) Expression profiling of three naive adult female Anopheles gambiae tissues: hemolymph (hemocytes), head and carcass (the remainder after hemolymph extraction and head removal).
Hemocyte response to Plasmodium berghei infection (Pinto et al., 2009) Gene expression in Plasmodium berghei infected Anopheles gambiae hemocytes was assayed with respect to infection with a midgut invasion-deficient CTRP knockout strain of the parasite.
Circadian rhythm: heads, dark-dark (Rund et al., 2011) A DNA microarray analysis of Anopheles gambiae under and constant dark (DD) conditions was undertaken to study the global regulation of genes by diel and circadian mechanisms. Adult mated, non–blood-fed female mosquitoes were collected every 4h for 48h, and RNA from heads was assayed on the Affymetrix Plasmodium/Anopheles microarray. For specialized cyclic expression analysis please see this external site.
Circadian rhythm: bodies, dark-dark (Rund et al., 2011) A DNA microarray analysis of Anopheles gambiae under constant dark (DD) conditions was undertaken to study the global regulation of genes by diel and circadian mechanisms. Adult mated, non–blood-fed female mosquitoes were collected every 4h for 48h, and RNA from bodies was assayed on the Affymetrix Plasmodium/Anopheles microarray. For specialized cyclic expression analysis please see this external site.
Circadian rhythm: heads, light-dark (Rund et al., 2011) A DNA microarray analysis of Anopheles gambiae under light/dark cycle (LD) conditions was undertaken to study the global regulation of genes by diel and circadian mechanisms. Adult mated, non–blood-fed female mosquitoes were collected every 4h for 48h, and RNA from heads was assayed on the Affymetrix Plasmodium/Anopheles microarray. For specialized cyclic expression analysis please see this external site.
Circadian rhythm: bodies, light-dark (Rund et al., 2011) A DNA microarray analysis of Anopheles gambiae under light/dark cycle (LD) conditions was undertaken to study the global regulation of genes by diel and circadian mechanisms. Adult mated, non–blood-fed female mosquitoes were collected every 4h for 48h, and RNA from bodies was assayed on the Affymetrix Plasmodium/Anopheles microarray. For specialized cyclic expression analysis please see this external site.
Adult tissues (Baker et al., 2011) This is the MozAtlas dataset. A number of tissues dissected from adult male and female Anopheles gambiae mosquitoes are assayed on the Affymetrix Plasmodium/Anopheles microarray. GEO: GSE21689
Desiccation stress (Wang et al., 2011) Gene expression in Anopheles gambiae G3 strain mosquitoes exposed to moderate and extreme desiccating conditions (70% and 30% RH respectively) was assayed on the Affymetrix platform. GEO: GSE25433