Bioinformatics Resource for Invertebrate Vectors of Human Pathogens
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Geneset Anopheles gambiae AgamP4.2 transcripts
Description A reference set of transcripts for the quantification of expression (FPKM) using Cufflinks. Visit https://www.vectorbase.org/organisms/anopheles-gambiae/pest/AgamP4.2
Accessions
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Experiments
Name Description
MOS55 cell-line infected vs uninfected (AVCL consortium, 2014) Transcription expression was compared between Anopheles gambiae cell-lines MOS55 infected with either the Wolbachia strains wMel or wMelPop, or a densovirus, and an uninfected cell-line. Data from the NIAID Arthropod Vector Cell line transcriptome (AVCL) consortium.
MR4 cell-lines (AVCL consortium, 2014) Transcription expression was compared between Anopheles gambiae cell-lines available from MR4 (Malaria Research and Reference Reagent Resource Center). Data from the NIAID Arthropod Vector Cell line transcriptome (AVCL) consortium.
Sua5B cell-line infected vs uninfected (AVCL consortium, 2014) Transcription expression was compared between Anopheles gambiae Sua5B cell-lines that have been infected with Wolbachia strains wAlb or wMel, and a control. Data from the NIAID Arthropod Vector Cell line transcriptome (AVCL) consortium.
Ivermectin-containing blood meals at different ages (Seaman et al., 2015) Gene expression was compared between blood-fed and non-blood-fed adult female Anopheles gambiae mosquitoes. Furthermore, the blood meals either contained 11.75 ng/ml ivermectin (solubilized in dimethyl sulfoxide and diluted in PBS) or the same volume of DMSO alone in PBS as control. The first blood meal was at two days post-emergence and a second blood meal was administered at six days post-emergence. The non-blood-fed control at six days did have the blood meal at two days. Mosquitoes receiving an ivermectin treated blood at six days were given an untreated blood meal at two days. All mosquitoes had ad libitum access to sugar and water except when being starved for several hours on blood-feeding days. Transcript expression was assayed via paired-end Illumina sequencing with quantification through a simple tophat2/cufflinks pipeline.