Where are the Culex EST libraries from
Answer:
The Culex ESt libraries have been generated by several laboratories:
- Janet Hemingway/Hilary Ranson, Liverpool School of Tropical Medicine, UK, made the normal libraries,
- Bruce Christensen, University of Wisconsin, made the bacterial infected libraries,
- Steve Higgs, University of Texas, made the West Nile virus infected libraries,
- Parakrama Karunaratne (now at University of Peradeniya, Sri Lanka) made the Filarial infected library at the Liverpool School of Tropical Medicine (UK),
- Peter Atkinson, University of California at Riverside, made the multi-stages library (pooled).
The libraries were sequenced by the Broad Institute and all the sequences were then submitted to dbEST.
Details of the multi-stages library:
- egg rafts,
- 1st instar larvae,
- 2nd instar larvae,
- 3rd instar larvae,
- 4th instar larvae,
- pupae,
- 10 young females (1 week old, pre-bloodmeal),
- young males (1 week old),
- 10 old adult females (1 week old, post bloodmeal),
- 10 adult males (1 week old).
Each life stage was extracted separately and sent to Invitrogen.
They pooled the samples so there was an equal representation of each life stage present.
