Champion et al. (2016) Anopheles gambiae mass spec. peptides

Whole head, compound eyes, total head appendages, and antennae proteomes of the Anopheles gambiae mosquito

Description of data

This study explored the protein composition of specific Anopheles gambiae mosquito head tissue isolates, with particular relevance to brain function, and the sensory modalities of vision, olfaction and gustation. We characterized the proteomes of isolated adult female An. gambiae mosquito tissues using a combination LC/MS/MS and targeted LC/MRM analysis; specifically whole mosquito head with all appendages attached, compound eyes, total head appendages (THAs; maxillary palps, antennae and proboscises), and antennae. Proteomics data were acquired on single-dimension LC/MS/MS runs using an LTQ Velos Orbitrap instrument (Thermo Fisher Scientific). The combined injections from Anopheles tissue yielded 8230 peptide identifications at a 1% false discovery rate (FDR) and 8350 at a 5% FDR, and 1,322 non-redundant protein identifications. Many of these identifications were subsequently validated using targeted LC/MRM based approaches; making this one of the largest representative collections of isolated head tissue protein identifications in mosquitoes to-date, with tissue specific quantification and detection.

The data, including the search results are also deposited into the PeptideAtlas with accession PASS00300.

Accessing the data


Champion M.M., Sheppard A.D., Rund S.S.C., Freed S.A., O’Tousa J.E. and Duffield G.E. (2015) Qualitative and quantitative proteomics methods for the analysis of the Anopheles gambiae mosquito proteome. Chapter 2 in ‘Short views on Insect Genomics and Proteomics’ book (Editors: T. Adeniran, R. Chandrasekar and M. Goldsmith), within the ‘Entomology in Focus’ book series (Editor: F.L. Cônsoli) (Springer publishers).


Experimentalists: Matthew M. Champion, Aaron D. Sheppard, Samuel S.C. Rund, Stephanie A. Freed, Joseph E. O’Tousa and Giles E. Duffield, University of Notre Dame, Notre Dame, IN, USA
VectorBase data integration: Bob MacCallum