As described in Holt et al. (2002: PMID 12364791), plasmid and BAC DNA libraries were constructed with stringently size-selected PEST strain DNA. Two BAC libraries were constructed, one (ND-TAM) using DNA from whole adult male and female mosquitoes and the other (ND-1) using DNA from ovaries of PEST females collected about 24 hours after the blood meal. Plasmid libraries containing inserts of 2.5, 10 and 50 kb were constructed with DNA derived from either 330 male or 430 female mosquitoes. For each sex, several libraries of each insert size class were made, and these were sequenced such that there was approximately equal coverage from male and female mosquitoes in the final data set. Celera Genomics, Genoscope and TIGR contributed sequence data that collectively provided 10.2-fold coverage, assuming a genome size of 278 Mb. The whole-genome data set was assembled with the Celera assembler to create this MOZ1 assembly, which constituted the basis of the primary genome publication (Holt et al. 2002). For this first draft, 91% of the genome was organized in 303 scaffolds, and scaffolds constituting about 84% of the genome have been assigned to chromosomes. No scaffolds were assigned to the Y chromosome. Automatic annotation was accomplished using two pipelines, both of which used ab initio gene-finding algorithms and relied heavily on diverse homology evidence to predict gene structure, Celera Otto genome analysis tools and the Ensembl group pipeline (European Bioinformatics Institute/Sanger Institute). A consensus gene set was created and manual annotation of 100 randomly selected gene models was done. The GenBank Record IDs are AAAB01000001 through AAAB01008987.

Genome Size (bp): 
Scaffold count: 
8 987
Release date: 
Wednesday, May 29, 2002


The Anopheles gambiae PEST strain was chosen for genome sequencing because it had both a fixed, standard chromosomal arrangement and a sex-linked pink eye mutation that could readily be used as an indicator of cross-colony contamination. The pink eye mutation originated in a colony called A. gambiae LPE established in 1951 at the London School of Hygiene and Tropical Medicine from mosquitoes collected in Lagos, Nigeria.

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