Johannesburg

The Johannesburg (JHB) strain is the source of genomic DNA for the Culex quinquefasciatus genome project. This strain has been selected because it consistently yields high quality chromosome spreads suitable for in situ hybridization studies. This strain is also insecticide susceptible and so can be transported and maintained in laboratories world-wide. The Johannesburg (JHB) strain was established from offspring reared from 20 egg rafts collected in a single unused fish pond in March 2001. Population genetic studies revealed that the Johannesburg C. quinquefasciatus population was genetically isolated from a sympatric C. pipiens pipiens population. Consequently, the JHB strain is expected to possess a high degree of genetic divergence from C. pipiens pipiens. Based on many generations of inbreeding at relatively small population size (often no more than about 50 females per generation), the colony is expected to show relatively little genetic variation. In addition to having excellent polytene chromosomes in larval salivary gland tissues, this colony has also been found to be free of any visible polytene chromosome inversions.

Note: As of May 2008, the subspecies Culex pipiens quinquefasciatus was promoted to species rank and is now named Culex quinquefasciatus. VectorBase changed this in August 2008. The NCBI taxon ID was maintained during this change.

Organism: 
Culex quinquefasciatus

The Culex pipiens complex is distributed worldwide and has two species formally recognized in the complex. One of these species is the tropical and subtropical C. quinquefasciatus (the southern house mosquito), vector of lymphatic filariasis and a number of arboviruses including St. Louis encephalitis virus and West Nile virus.

Genome assemblies and gene sets