Three types of WGS libraries were used to produce these Lutzomyia longipalpis sequencing data: a 454 Titanium fragment library and paired end libraries with 3 kb and 8 kb inserts. The 454 data (11.5 million reads; ~24.4x coverage) was derived from the same individual while mate pair reads (7.4 million 3kb reads, 9.6X; 3.7 million 8kb reads, 4.9X) were derived from a pool of individuals. In total about 22.6 million reads were generating representing 38.9x coverage of this sand fly genome.

The Llon_1.0 assembled draft genome sequence was built from the data described above using the Celera CABOG assembler (version 6.1, 2010/03/22). Next, these initial results were used as a backbone for longer superscaffolds using Baylor's ATLAS-link. Finally, discernible gaps filled with ATLAS-gapfill and ATLAS-gapmerge. The final assembly includes these superscaffolds, which can be ordered and oriented with respect to each other, and isolated sequences that could not be linked (single contig scaffolds or singletons from the original CABOG assembly).

The total length of all contigs is 142.7 Mb; however, the total span of the assembly is 154.2 Mb after gaps are included. The N50 of the contigs is 7.5 kb and the N50 of the scaffolds is 85.1 kb. Both the assembly and the description of the positions and orientations of contigs (AGP) are available from the Sand fly section of the BCM-HGSC web site at: www.hgsc.bcm.tmc.edu.

Sequence and Scaffold statistics:

Scaffolds/Contigs Number N50(kb) Bases+Gaps(Mb) Bases(Mb)
All Scaffolds 11,539 85.1 154.2 142.7
All Contigs 35,704 7.5 142.7 142.7
Deg Contigs 421,164 0.5 223.2 223.2

Genome Size (bp): 
Scaffold N50 (bp): 
13 854 319
Scaffold count: 
11 539
Release date: 
Wednesday, August 22, 2012


Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) is a new world sand fly. It was the first Lutzomyia species to be recognised as a vector of Leishmania in South and Central America. The female L. longipalpis transmits a protozoan parasite Leishmania infantum (also know as L. chagasi).

Gene sets

27 Jun 2017
21 Feb 2017
13 Jun 2015
25 Feb 2014
26 Apr 2013

Assembly Specific Downloads

 Downloads for this assembly